http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2001078756-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7952e8a492ee8ceb6e007e1f2bcbeb47 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0789 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-00 |
filingDate | 1999-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa2947db8f93355087a51c1f495fe85d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1bf5443afe460858e8d42699b38f56af |
publicationDate | 2001-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2001078756-A |
titleOfInvention | Washing method and washing solution for nucleated cells |
abstract | (57) [Summary] [Object] The object of the present invention is to enable centrifugal washing after thawing without generating clumps, regardless of the nucleated cell separation method used before cryopreservation, thereby reducing the cell recovery rate. It is an object of the present invention to provide a method for washing cells and a washing solution with a small amount of water. SOLUTION: When washing nucleated cells in a thawed nucleated cell-containing solution, a washing method comprising removing a cryoprotectant while suppressing cell aggregation. A washing solution for washing nucleated cells in a nucleated cell-containing solution after thawing, wherein the washing solution is a physiological solution containing at least a polysaccharide and / or a chelating agent. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013223504-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013168402-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-5196618-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-5276230-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2015232000-A |
priorityDate | 1999-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.