| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5547f741b25666fc4ae5195cf71a979b |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
1999-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d9bf53392e028ddfebae49acb22d03cd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06683472182052a9aa8f7e753cc73583
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5265e4b33c49c9472a399429ecf6aa0 |
| publicationDate |
2001-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2001011100-A |
| titleOfInvention |
Substrate for detecting HIV protease and vector for its expression |
| abstract |
(57) [Summary] [PROBLEMS] To detect a subtle change in a fluorescence signal, to facilitate production, and to determine the protease activity in a sample when the sample to be detected is a microorganism or a cell. Labeled substrate for proteolytic enzyme having a configuration enabling detection, vector for expression of the substrate, method for producing labeled substrate using the vector, and detection of proteolytic enzyme activity using the labeled substrate Providing a way. SOLUTION: DN encoding a labeled substrate for a proteolytic enzyme having a structure in which a fluorescent protein as a label is bound to each of an amino terminal and a carboxyl terminal of a peptide having a cleavage site by the proteolytic enzyme A labeled substrate is produced in a host by incorporating the A sequence into an expression vector and expressing it in a host such as a microorganism or a cultured cell. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8071167-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112683867-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112683867-A |
| priorityDate |
1999-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |