| abstract |
(57) [Problem] To provide a permanent mammalian cell line. A method for generating a mammalian cell line from primary mammalian cells, comprising: a) pretreating a culture of primary mammalian cells or a suspension thereof with at least one glucocorticoid; Obtaining a suspension of the pre-treated culture of step a), c) adding the pre-treated cells, not of retroviral origin, to the pre-treated cells of the suspension of step a) or b) A method is described which comprises the step of introducing at least one nucleic acid vector capable of immortalizing and further comprising the step of d) culturing the introduced cells in step c). Furthermore, there are provided mammalian cell lines and cells obtainable by the described methods, and pharmaceutical and diagnostic compositions containing such cells. |