| abstract |
(57) [Summary]nDisclosed is a human ubiquitin conjugating enzyme, designated hUBC-9, its complete amino acid sequence, and a nucleic acid polymer encoding hUBC-9. In addition to having functional ubiquinone binding activity, the enzyme has a transcriptional repressor activity that is independent of binding activity. The binding activity of hUBC-9 increases transcription due to degradation of transcription suppressor proteins such as WT1, or degradation of hUBC-9 itself. The repressor activity of hUBC-9 suppresses gene transcription, possibly by disrupting the transcription initiation complex due to specific interaction with the DNA binding region of TATA binding protein (TBP). Applications include hUBC-9, yUBC-9 and other ubiquinone conjugating enzymes with repressor activity that can be fused to a protein with a DNA binding domain such as Gal4, or the Wilms tumor suppressor gene product WT1 It can be used with a repressor such as In both pharmaceutical and non-pharmaceutical applications, such enzymes and the nucleic acid polymers that encode them can be used to regulate the transcription of target genes. |