http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2000514313-A
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-32 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-00 |
filingDate | 1997-10-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2000-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2000514313-A |
titleOfInvention | How to culture biomass |
abstract | (57) Abstract: Methylotrophic microorganisms are cultivated on methanol in a carbon medium as is known, and are continuously measured and post-fed to limit the methanol concentration to very low values. . According to the present invention, a culturing method comprising a plurality of culturing steps is carried out in a bioreactor equipped with a stirrer and an aeration tank by reducing the average methanol concentration from the first step to each of the following steps by a suitable feeding method. . In this case, the methanol supply is performed in proportion to the alkali solution supplied during the pH adjustment in the culturing step (c-2), and in the culturing step (c-1) at a specific supply amount and in the final culturing step (c). The first step is with a specific supply and then with a pO 2 -controlled supply. In this way, the accumulation of toxic intermediates is prevented. Measuring and adjusting the methanol concentration is not required for all culture stages. This significantly reduces the cost when implementing the method on an industrial scale. |
priorityDate | 1996-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.