http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2000506734-A

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filingDate 1997-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2000-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2000506734-A
titleOfInvention Recombinant method for producing cytochrome C (551) of Pseudomonas aeruginosa with Pseudomonas putida
abstract (57) Abstract: The present invention has ability to transfer electrons, the recombinant methods for producing the hemoprotein comprising the sequence of cytochrome C 551 Pseudomonas aeruginosa (Pseudomonas aeruginosa), the hemoprotein is Pseudomonas putida And a method characterized by being produced in The heme protein obtained by the method of the invention comprises native cytochrome C 551 and its precursors characterized by the presence of all or part of the signal sequence, typically the N-terminal part. The heme protein of the present invention can be produced by the following method. That is, (a) a host transformed with an expression vector containing a DNA sequence encoding a heme protein is provided under conditions such that the heme protein is expressed, and (b) the heme protein is isolated or purified. Accordingly, still another object of the present invention is to provide an expression vector comprising a DNA sequence encoding the heme protein of the present invention; a host transformed with a suitable expression vector according to the present invention; and encoding the heme protein of the present invention. Includes DNA molecules of natural or synthetic origin, including sequences. The heme proteins according to the invention find use in the diagnostic field, for example as chromogenic substrates for peroxidase or in electrochemical studies, for detecting, measuring and regulating the electron transfer reaction between redox proteins and electrodes. used.
priorityDate 1996-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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