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filingDate 1996-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2000-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2000500568-A
titleOfInvention Chemiluminescence analysis method and analyzer used for detection of an analyte
abstract (57) [Summary]nA sampling inspection device and method for quickly and easily detecting an analyte. The present apparatus and method perform highly sensitive detection using a chemiluminescence reaction, and can be used for applications such as detection of bacteria on the surface of an object. The inspection device has a sampling unit and a reagent unit. The sampling unit is made of an adsorptive material, and collects an analyte from a test sample such as an object surface or a liquid. The reagent part is made of an adsorbent material and contains one or more chemiluminescent reaction components such as luciferase enzyme and cofactor in a dry state. The device may have a movable shield to prevent contamination due to contact of the sampling section. It is preferable to wipe off the contaminated surface at the sampling section. Next, a lytic solution is added to the adsorbent to release ATP from bacteria collected as a sample. ATP diffuses into the reagent section of the device and reacts with cofactors such as luciferase contained in the reagent section. In response to bacteria present in the sample, luminescence by a chemiluminescent reaction occurs. This light can be electronically detected, for example, by inserting the device into a light detection box, or can be chemically detected by an arbitrary light detection unit formed of a film such as an instant photographic film.
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