| abstract |
(57) [Problem] To provide a simple method for measuring the activity of a phosphorylase that phosphorylates p53. The present invention relates to a DNA-dependent protein kinase. (DNA-PK), ATM, and phosphorylation of the substrate peptide that is phosphorylated by ATR are evaluated by an immunological method using an antibody that can identify the phosphorylation state, and phosphorylation (or dephosphorylation) of the substrate peptide is performed. A) providing a method for measuring enzyme activity; By applying this measurement method, it is possible to screen for compounds that regulate these enzyme activities. |