| abstract |
(57) [Problem] To provide a method for quantifying a substrate which can be applied to a single-use type biosensor and can eliminate at least variation in background current. SOLUTION: After a lapse of a predetermined time from the contact between the sample and the reaction layer, a working electrode potential E1 based on a counter electrode is applied to obtain a background current. A method for quantifying a substrate, comprising obtaining a response current at a potential E2 satisfying the following, and detecting the response current based on a difference between the response current and the background current. |