http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2000093172-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c6191997ed6dda6a5a3582e10b0b3dbc |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-02 |
| filingDate | 1998-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6893482111c794a8ad839015983570a6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_018f08939724fc0c9cd7755068caf438 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_08f95a0ebc44d34c7e3ea86b291686d2 |
| publicationDate | 2000-04-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2000093172-A |
| titleOfInvention | Method for producing recombinant antibody |
| abstract | (57) [Problem] To provide a new method for producing a recombinant antibody produced by genetic recombination using a microorganism as a host. SOLUTION: An antibody peptide produced by a recombinant host cell is solubilized, and a proper Cys-Cys bond is formed at the H chain peptide site and the L chain peptide site from a peptide solution obtained by dissolving the antibody peptide in a buffer. When rewinding to obtain a recombinant antibody molecule, the concentration of guanidine hydrochloride added to the peptide solution was initially higher than 3 M, and then the guanidine hydrochloride concentration was gradually reduced to 0 M by dialysis. Guanidine hydrochloride concentration At a stage of more than 0.5 M and less than 3 M, dialysis is performed on a solution containing a predetermined amount of oxidized glutathione, A method for producing a recombinant antibody, comprising performing the above-mentioned Cys-Cys bond formation and unwinding operations. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009297022-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008001802-A1 |
| priorityDate | 1998-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 73.