http://rdf.ncbi.nlm.nih.gov/pubchem/patent/IN-2012DE00119-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f337e6e0b48f6f01b7df6bccc4c6fa2d |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N- |
| filingDate | 2012-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cad93c4c961f720c1c3f16c660459196 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52af238c02e39195d7e4106d695d4387 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ae0229f81f029618511ebb00f936ec9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de60bb9b746a30135a214d86f9473170 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_171253f9a6c607a2071a88fb512b25d7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b794e26170820b85d7db2b85e213f634 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_53eeb2eed3ce7051ca19d1412efbb8ce |
| publicationDate | 2015-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | IN-2012DE00119-A |
| abstract | Abstract Real time detection of Enterococci in dairy foods using spore germination based bioassay The present invention relates to a spore germination based assay for the detection of microorganism Enterococci in a milk sample. The said assay comprises interaction of marker enzyme and non specific substrate resulting into the production of a germinant stimulus, which in turn indicates the presence or absence of target microorganism in a milk sample. Particularly, the invention enables the rapid detection of Enterococci which is based on targeting β-D-glucosidase as marker enzyme and its specific action on non specific substrate i.e. esculin / or cellobiose / or CMC resulting in germinant stimulus for spores produced by specific strain of B. megaterium. The assay is highly rapid and sensitive as the said assay enables the detection of target microorganism i.e. Enterococci in 8 ±2 hrs including sample enrichment in SAEBM (without esculin & ferric ammonium citrate) up to 4.5 ± 0.5 hrs and is capable of detecting the sample comprising as low as 5.5 ± 0.4 log cells. SAEBM has its application as selective media & micro-technique for detection and enumeration of Enterococci in milk sample. The assay time of micro-technique is ≤ 12 hrs with sensitivity up to 2-3 log count /ml. The invention also provides a sodium azide and esculin based culture media (SAEBM) which enables the selective enrichment of the target microorganism within 5-12 hrs. |
| priorityDate | 2012-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.