http://rdf.ncbi.nlm.nih.gov/pubchem/patent/IE-902467-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_36e8050eec40967e1cbc39ff89f2607d |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-708 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 |
| filingDate | 1990-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1991-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | IE-902467-A1 |
| titleOfInvention | Immobilised polynucleotides |
| abstract | Nucleotides can be linked from a 5'-phosphate residue to a support via a dithio (-S-S-) linkage. Polynucleotides thus immobilised can be used in the polymerase chain reaction (PCR) at which a temperature of 90 DEG C is typically required. The -S-S- linkage can be produced by reaction of a thiolated support with a 5'-(thiol-terminated) polynucleotide. For ease of hybridisation of the immobilised polynucleotide, the sequence to be hybridised should be adequately spaced from the support. The invention is useful in any situation in which a polynucleotide such as DNA is required to be separated as a solid phase from a liquid phase, especially in heterogeneous assays and to isolate products of the PCR. |
| priorityDate | 1989-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 94.