| abstract |
New antigenic peptides (I) comprises all or part of the amino acid sequence of the melanoma-associated p97 antigen Also claimed are recombinant viruses contg. a DNA sequence coding for (I) under control of an expression-regulating DNA sequence, and recombinant DNA vectors (plasmids) p97b, pMtp97b and pSVp97a. p97b pref. comprises a 4.4 kb insert of the p97gene (from SK-MEL28 melanoma) in pEMBL18+. Excision of this insert and insertion into the SmaI site of the eukaryotic cDNA expression vector 1995.12pUC13 yields pMRp97b, which is used to transform LMTK-cells to obtaina p97-producing transformant TKMp97-12.n[CH675424A5] |