| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_32054995c5e8124190a7f9bd976b205c |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2458-10 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2014-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ff25ed87a9185c1995a58ca8986add7f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8834c3b1fd916bc1db6fed3e4c6ab142
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f5812411074feb972ced2c5a34e59d4e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f9fc4662c2d8134ff5bc92140f76f4d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5286ff575b5f739c65612aead9290eb9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28249d2467f4a6411b94ddb8d37aa9fe
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_226c39c79934f21ac2e7cab0d6c0b543
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ce3fb54fad039b056f0e780399383466
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5769716743dbc3c8bace8c79df5e46be |
| publicationDate |
2016-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
IE-86624-B1 |
| titleOfInvention |
Methods and systems for quantitative fluorescence-based detection of molecules and proteins |
| abstract |
A kit and method for detection of a target in a sample. An assay mixture provided in the kit and used in the method includes a first probe with a first antibody recognizing a first epitope of the target and conjugated to an RNA oligonucleotide; a second probe with a second antibody recognizing a second epitope of the target and conjugated to a DNA oligonucleotide; a reverse primer with a first region complimentary to the RNA oligonucleotide and a second region complimentary to the DNA oligonucleotide; and a reverse transcriptase that creates a DNA transcription product from the RNA oligonucleotide using the reverse primer only if the RNA oligonucleotide and the DNA oligonucleotide are in close proximity. If the target is present in the sample, the reverse primer binds the RNA oligonucleotide and the DNA oligonucleotide to bring the RNA oligonucleotide and the DNA oligonucleotide in close proximity. |
| priorityDate |
2013-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |