http://rdf.ncbi.nlm.nih.gov/pubchem/patent/IE-810680-L
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2ca2e4410855da087e11bf3eea8933fa |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-067 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-62 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C231-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-575 |
filingDate | 1981-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 1981-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | IE-810680-L |
titleOfInvention | Insulin |
abstract | A process for the production of insulin or an insulin analog is provided by combination of an A-chain and a B-chain, which comprises reacting the S-sulfonated form of the A-chain, the S-sulfonated form of the B-chain, and a thiol. reducing agent together in an aqueous medium under conditions which produce a mixture having (1) a pH of from about 8 to about 12, (2) a total protein concentration of from about 0.1 to about 50 milligrams per milliliter, and (3) an amount of thiol reducing agent which affords a total of from about 0.4 to about 2.5 -SH groups per each -SSO3<-> group present in the total amount of A- and B-chain S-sulfonates, and allowing formation of insulin or an insulin analog to occur by maintaining the mixture at a temperature of from about 0 DEG C. to about 25 DEG C. and in an environment which provides a source of oxygen.n[EP0037256A1] |
priorityDate | 1980-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 103.