| abstract |
The invention relates to a synthetic gene which contains in the coding strand codons for the amino acids of a required peptide in tandem repeats. The invention further comprises a process of a synthetic gene of this type, which is characterised by self-attachment and ligation and, where appropriate, repair of a plurality of suitable oligonucleotides, where the attachment is achieved with the formation of the repetitive character by self-complementary, overlapping ends at the termini of the group of oligonucleotides which, after the attachment, form the repetitive unit of the gene. A synthetic gene of this type can be used to prepare recombinant plasmid vectors, to transform cells and thus to express peptides. The invention is applicable, inter alia, to the preparation of (asparagyl-phenylalanine)n and, from this, the sweetener aspartame, that is to say the methyl ester of asparagyl-phenylalanine.n[FR2479259A1] |