http://rdf.ncbi.nlm.nih.gov/pubchem/patent/IE-35076-L
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d202e9e866984fe229f6facc38f1d740 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1258 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-12 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 |
| filingDate | 1971-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1971-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | IE-35076-L |
| titleOfInvention | PRODUCTION OF PNPase |
| abstract | The enzyme (PNPase) is obtd. by (a) suspending a cell mass separated from a Micrococcus lacteus fermentation medium in aqs. saline soln., and homogenising or lysing the suspension to rupture the cells to give a suspension of insol. cell debris in an aqs. PNPase soln.; (b) adding (NH4)2SO4 to a concn. of approx. 24% and centrifuging to remove the cell debris and some protein material; (c) adding to the supernatant of aqs. PNPase sufficient lower alkanol to ppt. almost all of the PNPase, and centrifuging the aqs. alcoholic mixt. to recover the ppted. enzyme. PNPase catalyses the polymerisation of nucleoside diphosphates to polynucleotides. For use as a catalyst, the enzyme is covalently bound to activated cellulose.n[FR2091158A5] |
| priorityDate | 1970-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 177.