http://rdf.ncbi.nlm.nih.gov/pubchem/patent/HU-226260-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_649359fbda1c071415fec733a1579db2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9b9f6fa365773a759b24e6009fd3ec72 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-52 |
| filingDate | 2002-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_188018f2be9cf482c6b98383fb718285 |
| publicationDate | 2008-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | HU-226260-B1 |
| titleOfInvention | Process for determination of concentration of n-epsylon (gamma-glutamyl) lysine-isodipeptide and n-epsylon (gamma-glutamyl)lysine-isodipeptide binding containing peptides, proteins and kit for thereof |
| abstract | FIELD OF THE INVENTION The present invention relates to a method for determining the concentration of peptides and proteins containing Ne (γ-glutamyl) lysine isodipeptide and Ne (γ-glutamyl) lysine isodipeptide bonds. The method is characterized by measuring a mono-or polyclonal antibody to Ne (Y-glutamyl) lysine isodipeptide in a physiological saline solution or buffer solution to the wells of a plate having a high protein binding coating and binding it to the coating on the plate at room temperature and then known as Ne (y- a dilution series is prepared from a standard solution containing a compound which is chemically bound to the isopeptide, preferably biotin, and is prepared from a standard solution of the given concentration and from the solution to be determined, which is filled into the measuring wells of the measuring plate, incubated, then the non-antibody-bound NE (γ-glutamyl) lysine isodipeptide is washed, then the enzyme solution, preferably phosphatase solution, bound to the standard compound in the standard solution is weighed into the wells and the solution is incubated and then resuspended. the agonized enzyme buffer solution is washed, then the amount of enzyme activity bound to the site is measured and the isopeptide concentration to be determined is calculated by comparing the enzyme activity values of the standard solution. The invention further relates to a kit for carrying out the method. Scope of the description is 6 pages (including 1 page figure) |
| priorityDate | 2002-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.