Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bb12c8e7bcdb4d4fce1a3c957db87dd5 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-822 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A62D2101-06 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C02F3-342 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0044 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B09C1-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A62D3-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C06B21-0091 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C06B21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C02F3-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B09C1-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12S99-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A62D3-02 |
filingDate |
1996-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2fbbbd7d4052270c5342181f82fcba23 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7e3a644edff4279f1f3cf61fb25c65c8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bbc7d4fba2469ad36c08d19698853ed5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f62b3483c41185873ec546671effdaee |
publicationDate |
2005-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
HU-224000-B1 |
titleOfInvention |
Detection and biodegradation of explosives |
abstract |
The present invention relates to an enzyme catalyzing the removal of nitrite from pentaerythritol tetranitrate (PETN), the enzyme PETN reductase. Azenzyme was produced by culturing a new strain of Enterobacter cloacae isolated from nature. The strain designated PB2 was deposited under NCIMB-40 718. The amino acid sequence of the enzyme and the gene encoding the enzyme (onr gene) have also been determined. The invention thus relates to an isolated pentaerythritol tetranitrate reductase (PETN reductase) enzyme which catalyzes the removal of nitrite from pentaerythritol tetranitrate (PETN) and a derivative thereof, as well as a gene encoding the enzyme. The invention further relates to processes for the large-scale production of PETN reductase enzyme and its use in biological recovery. In addition, the present invention provides a method for detecting the presence of PETN in a sample and a biosensor useful in such a method. Thus, the present invention is suitable for the preparation of a large batch of the enzyme according to the invention and for the purification of the environment contaminated with PETN and related compounds. The present invention is suitable for the detection of small amounts of PETN. ŕ |
priorityDate |
1995-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |