http://rdf.ncbi.nlm.nih.gov/pubchem/patent/HU-221236-B1

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publicationDate 2002-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber HU-221236-B1
titleOfInvention Process for microbiological hydroxylation of gonene-derivates
abstract The present invention relates to a process for the preparation of 15? -Hydroxy-13? -Ethyl-4-gonene-3,17-dione from 13? -Ethyl 4-gonene-3,17-dione with a fungal strain having steroid hydroxylase enzyme activity. consists of the identification of Fusarium nivale sp. VJ-63 or NCAIM F 001211 Fusarium nivale sp. VJ-90 or NCAIM F 001209 with Fusarium moniliforme sp. Mortierella pusilla sp. VJ-104 or NCAIM F 001208 identification mark. The VJ-20 fungus strain, optionally induced with Norrettonone, is grown on an assimilable source of carbon and nitrogen, and in mineral medium-containing media, in recycled, aerated conditions at 24-28 ° C for growing cultures 12-40. at 13 hours, the culture to be transformed is continued by bioconversion for 48-120 hours, the main product being 15? -hydroxy-13? -ethyl-4-gonene-3,17-dione and the mixture of 7?, 15? -dihydroxy-13? -ethyl-4-gonene-3,17-dione formed as a by-product is isolated from the fermentation broth in a manner known per se, and then separated by chromatography. The invention further relates to the novel 7,15,5-dihydroxy-13-ethyl-4-gonene-3,17-dione. ŕ
priorityDate 1996-08-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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