abstract |
A method for producing a protein that is naturally gamma carboxylated, properly folded, and processed and wherein the protein is encoded in a recombinant DNA vector such that the protein is expressed when a eukaryotic host cell containing the vector is cultured under suitable expression conditions, which comprises:n (a) inserting the vector into an adenovirus-transformed, human embryonic kidney cell; and (b) culturing said host cell of step a) under growth conditions and in media containing sufficient vitamin K for carboxylation. |