| abstract |
Novel bifunctional recombinant DNA cloning vectors and vector transformants opt. contg. transposon and antibiotic-resistance determining genes are prepd. by coupling DNA fragments carrying antibiotic-resistance determining genes contg. functional replicant oligo in E. Coli cells and DNA fragments contg. functional replicant oligo in Streptomyces cells and opt. transposon DNA, transforming Streptomyces and E. Coli cells with the resulting recombinant vector DNA, selecting the transformants and opt. isolating the mutants. |