abstract |
The present invention provides recombinant constructs for reducing the expression of endogenous mRNA and any substantially identical endogenous RNA, and methods for reducing the expression of target mRNAs and identifying or screening for essential plant genes. Among other things, a recombinant construct comprising a suitable nucleic acid sequence and a reverse complement thereof can be used to alter the expression of any homologous endogenous RNA (i.e., target RNA) that is in the vicinity of a suitable nucleic acid sequence. SHE |