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filingDate 2001-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eeb64556ab63aa992168f28813d3015b
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publicationDate 2003-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber HU-0302232-A2
titleOfInvention Equipment and Methods for Separation of X-Chromosome Sperm Cells and Y-Chromosome Sperm Cells, Process for Particle Differentiation and Particle Classification Equipment
abstract FIELD OF THE INVENTION The present invention relates to an apparatus and method for separating chromosomes carrying chromosomes X and spermatozoa bearing Y chromosomes, method for separating particles and a particle sorting device. For the purpose of a method of separating spermatozoa and chromosomes bearing chromosome Y carrying the X chromium atom of the invention, male cells are collected from the mammal of a mammalian species; determine the sex-determining characteristic of sperm cells; distinguishes sex cells based on the gender-determining characteristic; separating the distinguished sperm cells into a population carrying the Xchromosomes (70, 71) and the chromosome of the Y chromosome; and producing at least 90% pure, isolated X-chromosomal or Y chromosome-bearing male cell populations (70.71). The X-chromosome-containing spermatozoon and Ykromosome-bearing sperm cells have a spleen cell that has a different sperm cell volume in the case of sperm cells carrying the X chromosome or carrying the Y chromosome; includes an electromagnetic beam of the initial waveform which reacts differently to the difference between the volume of the chromosome carrying the X chromosome and the carrier chromosome Y; a detector responsive to the modified waveform of the electromagnetic beam; and a detector-related analyzer for determining the difference between the volume of sperm cells carrying chromosome X and the volume of sperm cells bearing the chromosome Y. A method for distinguishing particles, the method comprising collecting asymmetric particles; providing a fluid stream with a flow cytometer; introducing asymmetric particles into a liquid stream; orient the asymmetric particles relative to a single detector; depending on the orientation of the asymmetric particles to the detector-oriented orientation, a light having a different characteristic is detected; converting light having a response responsive to the asymmetric particle orientation to information about the orientation of the asymmetric particle less than one signal; analyze information about asymmetric particle orientation; and determining the orientation of the asymmetric particle relative to the detector. The particle classification device comprises at least one asymmetric particle having an orientation characteristic located in a fluid stream; comprising a source of radiation responsive to the asymmetric particle; includes a beam focusing optic; a beam-emitting light-emitting material-emitting material associated with the beam; the light emitting material is the particle
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