| abstract |
The present invention provides a stereoselective enzymatic conversion of certain keto-carboxylic acid derivatives to the corresponding alkylamino compounds. According to the invention, an example of 5- (1,3-dioxol n-2-yl) -2-oxo-pentanoic acid is converted to the 5- (1,3-dioxol n-2-yl) reductive amine as shown below. ) -2S-Amino-pentanoic acid (L-allisin-ethylene-acetyl): wherein R 1 is hydrogen or lithium. The microorganism (e.g. ul Pichiapastoris), which is capable of producing in situ amino acid dehydrogen, preferably phenylalanine dehydrogen, and a single enzyme such as formaldehyde dehydrogen, which regenerates an acofactor, is used. The advantage of the procedure over single-step chemical synthesis is the one-step reduction specific for the S-enantiomer. SHE |