| abstract |
The present invention relates to methods for identifying gene sequences of potential vaccine antigens. The present invention relates to the use of the gene sequences and polypeptides encoded by the gene sequences and to the use of such sequences in inducing a protective immune response in animals. In particular, the invention relates to the identification of potential antigenic sequences of Mycoplasma, preferably Mycoplasma hyopneumoniae. In one aspect, the invention relates to a method of expressing expression proteins translated from a nucleotide sequence in an expression vector, which method comprises co-expressing the protein translated from the nucleotide sequence and a marker. In another aspect, the invention provides a method of identifying a therapeutic antigenic gene encoding a therapeutic antigenic protein of a disease from a nucleotide sequence sample. The marker is preferably a poly-His tag. SHE |