http://rdf.ncbi.nlm.nih.gov/pubchem/patent/HR-P20100011-T1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_251f2686051db450ca11541fe3de499d |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-95 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-90 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-7151 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K19-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 2010-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6ecbe06306b6e63ad006a53e037b9309 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3cf752ae9c7f38d07c91f48431d624e9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_107e248ec6e12adfa946e8c621893fb5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7764a0d8eaf29c4a4808d0a8ecc658c1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_266a6a435de655f56beff4c24bbe2e29 |
| publicationDate | 2010-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | HR-P20100011-T1 |
| titleOfInvention | PROCEDURE FOR THE PRODUCTION OF TNFR-IG FUSION PROTEIN |
| abstract | A method for producing a tumor necrosis factor and immunoglobulin (TNFR-Ig) receptor fusion protein in bulk cell culture, comprising the steps of: obtaining a cell culture comprising: mammalian cells comprising a gene encoding the TNFR-Ig gene which is expressed under cell culture conditions; glutamine-containing imedium having the characteristics of a medium selected from the group consisting of: (i) a cumulative amount of amino acids per unit volume greater than about 70 mM, (ii) a molar ratio of cumulative glutamine to cumulative asparagine of less than about 2, (iii ) molar ratio of cumulative glutamine to cumulative total amino acids less than about 0.2, (iv) molar ratio of cumulative inorganic ions to cumulative total amino acids between about 0.4 and 1, (v) combined cumulative amount of glutamine and asparagine per unit volume about 16 mM, and combinations thereof; maintaining said culture in the initial growth phase, under a first set of culture conditions, during a first period of time sufficient to allow said cells to reproduce to a vital cell density within the range of about 20% -80% of the maximum possible vital cell density, when said culture is maintained under the first set of cultural conditions; modifying at least one culture condition so that a second set of culture conditions is then applied; maintaining said culture during the second period of time under this second set of conditions, and in the second period of time TNFR-Ig accumulates in the cell culture. The patent contains another 49 patent claims. |
| priorityDate | 2004-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.