http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-977327-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2269802eb012f5414c8655a375a042a4 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-465 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-886 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H13-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P1-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H13-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P1-06 |
| filingDate | 1961-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1964-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-977327-A |
| titleOfInvention | An antibiotic moenomycin and a process for its manufacture |
| abstract | <PICT:0977327/C1/1> The antibiotic Moenomycin is prepared by cultivating an antibiotic Moenomycin-producing strain of Steptomyces bambergiensis, Streptomyces ghanaersis, Strepromyces ederensis or Streptomyces geysiniensis under aerobic preferably submerged conditions in an aqueous nutrient medium containing a source of carbon and of nitrogen with or without an inorganic salt until a substantial antibiotic activity is imparted to the medium and isolating the antibiotic Moenomycin from the medium. The antibiotic is isolated from the culture solution by extraction with an organic solvent e.g. butanol at pH 2-3 or by means of an adsolvent and from the mycelium by extraction with a water-miscible or partially miscible organic solvent e.g. methanol, acetone or dioxan, or by destruction of the cells by deep freezing or thawing, or by homogenizing, with water or aqueous solutions of salts, or organic or inorganic acids. The evaporated solvent extracts are extracted with hot polar organic solvents and the antibiotic is precipitated from the concentrated extract by a non-polar organic solvent e.g. diethylether or ethylacetate. The antibiotic can also be isolated from the mycelium extract by (a) precipitation from the aqueous residue remaining after evaporation of organic solvent, with mineral acid at pH 1-5, (b) extraction of the acidified aqueous residue with a solvent which is non-miscible or only partially miscible with water and (c) adsorbing the aqueous residue on bleaching earths and eluting with aqueous organic solvents. The antibiotic is purified by adsorption method (c) and counter current distribution in a butanol/water system. The antibiotic Moenomycin is a weak acid and forms metal salts with alkali metal (e.g. sodium) hydroxides and has negative ninhydrin, biuret, fehling, Ehrlich, Sakaguchi, aniline phthalate and ferric chloride reactions. The antibiotic Moenomycin is colourless; has the elemental analysis C=50.4%, H=7.3%, N=6.3%; I.R maxima at 3.02, 3.45, 6.03, 6.50, 7.28, 7.55, 8.20 and 9.38 m as shown in Figure; decomposes at 200 DEG C.; in paper electrophoresis shows no migration in buffer solutions of pH 1.9 to 7.8 and anodic migration in buffer solution at pH 7.8; solubility in water, alcohols of 1 to 4 carbon atoms and polar solvents e.g. pyridine and sparing solubility in alcohols of more than 4 carbon atoms and insolubility in diethylether, and other non-polar solvents; stability in neutral aqueous and methanolic solutions and instability in acid and alkaline solutions; and no U.V. absorption maxima in the range 220-400 mm Various Rf values are given. The antibiotic Moenomycin or its physiologically tolerable metal salts may be use in pharmaceutical compositions e.g. as an injectable solution. |
| priorityDate | 1960-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.