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filingDate 1963-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 1964-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber GB-965056-A
titleOfInvention Microbiological nitrogen fixation
abstract A fertilizer is produced by growing a micro-organism in an aqueous nutrient medium in the presence of nitrogen, oxygen, and a hydrocarbon. The micro-organism may be Bacterium nitrimethanicum, Pseudomonas nitrimethanica or Pseudomonas nitrimethanica var. citreus. The hydrocarbon may be methane, propane, n-butane, n-hexane, n-heptane, kerosene, or ethyl benzene. The nutrient medium may contain sodium, potassium, manganese, and iron cations; and carbonate, chloride, sulphate, phosphate, and molybdate anions. The temperature may be 75-115 DEG F. The pH may be 6-8. The hydrocarbon, nitrogen and oxygen may be in the form of a hydrocarbon/air mixture containing 15-50% of hydrocarbon. The micro-organism may be grown in a sparger (Fig. 1, not shown) or in the soil. The proportion of hydrocarbon to soil may be 0.01-0.1%. A liquid hydrocarbon may be sprayed on the soil. A gaseous hydrocarbon may be fed into the soil through perforated plastic pipes.
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