http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-928681-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a1d54c08eeb8540176be204d4fb87ef3 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-35 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-35 |
| filingDate | 1959-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e37b80a03f67ddc03a165cce315d0119 |
| publicationDate | 1963-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-928681-A |
| titleOfInvention | Improvements in or relating to the extraction of corticotrophin |
| abstract | Corticotrophin is obtained from fresh or acetone-dehydrated animal pituitary glands by extraction with an aqueous, acid, organic solvent mixture containing a major proportion, e.g. 65-80%, of a water-miscible aliphatic alcohol and/or ketone, e.g. ethanol or acetone, the solvent mixture being heated during at least a part of the extraction operation to a temperature at which at least some of the inert proteins are denatured. The pH of the solvent mixture may be between 1 and 3 and is preferably about 1.5. After extraction, the supernatent may be decolorized by contacting with charcoal which has previously been treated with a reducing gas, e.g. H2S, the pH being maintained below about 2. Further purification is effected by the selective precipitation of protein by the addition of a water-soluble zinc salt, e.g. the acetate or chloride and the adjustment upward of the pH by addition of caustic soda solution. Below pH 4.45 the unwanted protein is precipitated and may be removed. Continued upward adjustment causes the almost quantitative precipitation of a zinc-corticotrophin complex between pH 4.45 and about 5.85. This may be collected, e.g. by centrifugation at low temperature (0 DEG C.), washed and decomposed by acidification to pH 3.5-4.5 to give a solution of corticotrophin from which, after filtration, it may be adsorbed by a mixture (preferably 1 : 1) of oxycellulose and a cation-exchange resin having functional carboxylic acid groups and subsequently eluted by treatment with 0.1 NHCl. The purified corticotrophin may then be precipitated from the eluant by the addition thereof to a large volume of cold acetone. Specification 928,682 is referred to. |
| priorityDate | 1959-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.