http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-853232-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_36fbd564ac0af5b3c3b77ce6dfa14fe4 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H19-20 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-20 |
| filingDate | 1957-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1960-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-853232-A |
| titleOfInvention | Improvements in or relating to coenzyme a |
| abstract | A stable metal salt reaction product of coenzyme A is obtained by reacting coenzyme A with a water-soluble non-deliquescent metal salt in aqueous solution and then lyophilizing the reaction mixture. The metal salt is suitably a metal salt of a divalent acid and may be a salt of an organic acid or of a mineral acid such as hydrochloric acid. It is preferably a divalent metal salt of a dibasic organic acid. Specified salts are the salts of calcium, copper (cupric), magnesium, cadmium, nickel (nickelous), cobalt (cobaltous), iron (ferrous) and manganese with gluconic, benzoic, citric, acetic, formic, lactic, succinic, tartaric, hydrochloric, sulphuric, and nitric acids in so far as such salts are watersoluble and non-deliquescent. The reaction is preferably carried out by mixing the two reactants in aqueous solution at room temperature and then lyophilizing the solution thus obtained. The product after lyophilization is enzymatically stable and has the same spectrophotometric and chromatographic characteristics as pure coenzyme A stabilized by cold. In aqueous solution the product decomposes by hydrolysis to give coenzyme A. A stoichiometric excess of the metal salt may be used in the reaction. Examples are given in which coenzyme A is reacted with calcium gluconate, calcium benzoate, and calcium citrate respectively in aqueous solution. The solution is then filled into ampoules which are placed in a refrigerator and frozen to-70 DEG C. in 15 minutes. The mixture is then evaporated in vacuo over 10 hours with progressive increase of temperature to about 20 DEG C. and the ampoules are finally filled with nitrogen and sealed. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7348315-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6255292-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5712258-A |
| priorityDate | 1956-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 47.