http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-849508-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8164846b58b60d95789a38989e5cfce8 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2428 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-34 |
| filingDate | 1958-03-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1960-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-849508-A |
| titleOfInvention | Purification of amyloglucosidase and preparation of dextrose |
| abstract | Amyloglucosidase (alpha-glucosidase, glucamylase or starch-glucogenase) is purified by adding to a 50% by volume aqueous acetone solution of crude amyloglucosidase 0,25 gram. of a water-soluble inorganic neutral salt such as sodium chloride, potassium chloride or sodium sulphate per 100 ml. of the acetone solution, to precipitate the amyloglucosidase separating and washing the amylcoglucosidase, re-dissolving in 50 % by volume of aqueous acetone, adding 0,25 grm./1 ml. of said salt to precipitate the amyloglucosidase which is separated and may be dissolved in water for use in the production of dextrose from starch (see Group VI). The crude amyloglycosidase is an aqueous extract or culture filtrate of a fungus such as Aspergillus niger. If sufficient, electrolyte is present in the aqueous extract, acetone is added to produce 50% acetone without the further addition of electrolyte. This may be given a preliminary treatment consisting of (a) centrifugation or (b) adsorption on a water-insoluble hydrous synthetic magnesium silicate in accordance with Specification 849,509.ALSO:A process of producing dextrose from starch wherein the starch in aqueous solution is hydrolysed with amyloglucosidase, the hydrolysate clarified, concentrated, and the crystallised dextrose separated from the mother liquor, characterised in that the amyloglucosidase solution has been purified by repeated precipitation from 50% aqueous acetone by the addition of a p water-soluble inorganic salt electrolyte such as sodium chloride (see Group IV(b)), the final hydrolysate having maximal DE values of 93 to 96% and maximal true dextrose content of 90 to 98% dry basis. An initial purification of the amyloglucosidase may be by the adsorption process of Specification 849,509. It is preferred to use a pure starch which has been subjected to a preliminary acid hydrolysis so that a workable fluid is obtained at a solids concentration of 25 to 50%, and especially 35%, at 45 to 60 DEG C. A pH of 4 to 5 and a temperature of 35 to 45 DEG C, is preferred. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112522113-A |
| priorityDate | 1957-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.