http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-823516-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_242525e02da3301c3e4f8c24683b36d3 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H15-234 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H15-234 |
| filingDate | 1958-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1959-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-823516-A |
| titleOfInvention | Purification of kanamycin |
| abstract | Kanamycin is recovered from aqueous solutions such as a fermentation broth or in exchange resin eluates, by extraction at an alkaline pH, namely pH above 7 to 11 and especially 8.5 to 10.5, with a lesser volume of a water-immiscible 1 to 6 alkanol containing, per mol. of kanamycin in the aqueous solution, from one to ten mols. of an unsubstituted aliphatic aldehyde containing from 4 to 10 C atoms inclusive or a monocyclic aromatic aldehyde, separating the alcohol phase and subsequently extracting the kanamycin in the alcoholic solution of kanamycin at an acid pH is below pH 4.0 into a lesser volume of aqueous acid and separating the concentrated aqueous solution of kanamycin thus obtained. The preferred alkanol is n-butanol, although pentanol and hexanol may be used and at least four molecules and especially 4 to 8 mols. of aldehyde per mol. of kanamycin is used. The alkanol is used in quantities of less than 1/4 and preferably 1/2 of the aqueous kanamycin solution. The aqueous acid is used in quantities of less than 1/4 and preferably 1/20 of the volume of the alkanol extract. To recover kanamycin in the case of a sulphuric acid extract, the pH is adjusted to pH 8.2 by the addition of ammonia and methanol added to precipitate kanamycin sulphate. The aldehydes may be (a) substituted and unsubstituted monocyclic aromatic aldehydes, e.g. furfuraldehyde, cinnamaldehyde, 5-nitrofurfuraldehyde, 2-, 3-, and 4-formylpyridine, 2-and 3-formylthiophene, benzaldehyde, alkyl benzaldehydes such as p-methylbenzaldehyde and p-isopropyl benzaldehyde, alkoxybenzaldehydes such as anisaldehyde and veratraldehyde, halobenzaldehydes such as p-chlorobenzaldehyde, hydroxybenzaldehydes such as vanillin and salicyclaldehyde and alkylaminobenzaldehydes such as p-dimethylamino benzaldehyde, and (b) n-butyraldehyde, isobutyraldehyde, n - heptaldehyde, 2 - ethyl hexanol, capraldehyde, n-valeraldehyde and isovaleraldehyde. |
| priorityDate | 1958-01-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 77.