http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-760089-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6a8240e48f43839cef5ed0f365d376a9 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0065 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-08 |
filingDate | 1954-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 1956-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | GB-760089-A |
titleOfInvention | Improvements in or relating to the preparation of catalase |
abstract | A method of preparing a stable water-soluble amorphous catalase of high potency comprises extracting catalase-bearing tissue, e.g. comminuted fresh or frozen mammalian livers or kidneys, with a 20 to 30 per cent by volume aqueous acetone solution at a temperature of 15 DEG to 30 DEG C. for at least 8 hours, increasing the acetone concentration of the extract to between 30 and 40 per cent by volume to precipitate water-soluble contaminants and increasing the acetone content of the separated supernatant liquid to between 45 to 55 per cent by volume to precipitate the catalase together with water-insoluble contaminants. The precipitate may be dried in a vacuum oven at approximately 45 DEG C. and used in the crude form, or purified by extraction with water, and clarification of the separated extract, e.g. by filtration or centrifugation, prior to concentration to a stable concentrated solution, or lyophilization to yield a stable dry powder. |
priorityDate | 1953-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 161.