http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-700715-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6a8240e48f43839cef5ed0f365d376a9 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6481 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64 |
| filingDate | 1951-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e46031ebfed82e9dbbb0c221c29705d1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5698c0d88da80af9fadfb75c35aeb715 |
| publicationDate | 1953-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-700715-A |
| titleOfInvention | Preparation of pepsinogen and pepsin |
| abstract | Pepsinogen is extracted from sources containing it by treatment at -10 DEG C. to + 5 DEG C. with an alkaline solution containing an aliphatic water-miscible solvent for pepsinogen having 1-5 carbon atoms. Pepsinogen is precipitated from the resulting extract by the addition of a protein-precipitating acid or a salt of a di- or poly-valent metal. If desired it may be converted into pepsin by treatment with an acid, or else pepsinogen may be obtained from the precipitate. Suitable conditions for the extraction are a temperature of about -5 DEG C. and pH of 7-8.5, using acetone, methanol, ethanol, propanol or ethylene glycol as the solvent. The preferred solvent is 40-50 per cent. ethanol. The precipitating agent may be a salt of calcium, barium, magnesium, iron or other heavy metal, or trichloroacetic, tannic, picric or sulphosalicylic acid. Pepsin may be obtained from the precipitate by acidifying to below pH 4.5, warming to 25 DEG C., filtering, and evaporating or precipitating with ethanol and acetone. In the examples, (1) ground hog stomach linings are treated with aqueous ethanol at -3 DEG C. and pH 7.4 and the extract separated by centrifugation, (2) as in (1) at -4 DEG C. and pH 7.8, the extract being separated by filtration; trichloracetic acid in aqueous ethanol is then added at -5 DEG C. and the precipitate separated by centrifugation, (3) as in (2) using ferric chloride as precipitant, (4) as in (1) at -3 DEG C. and pH 8.0, the extract being separated and precipitated with calcium acetate, (5) as in (4) at -5 DEG C. and pH 7.6, (6) the precipitate from (5) is treated with sodium chloride and hydrochloric acid to bring the pH to 2.0 and warmed to 20 DEG C.; the solution is clarified and dried. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5340737-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02069736-A1 |
| priorityDate | 1951-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 65.