http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-691501-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_25f5e60cca882cd756ee1cd36c3fef16 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2408 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-26 |
| filingDate | 1951-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1953-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-691501-A |
| titleOfInvention | Preparation of hyaluronidase |
| abstract | The invention comprises a method for the concentration and purification of hyaluronidase starting with an aqueous solution thereof free from tissue and containing an acid buffer system at pH 4.0-5.0 and strong electrolyte to make the ionic strength 0.1-0.25; such a solution is cooled to 0 DEG C., alcohol is added to give a concentration of 30-40 per cent by volume, when some hyaluronidase is precipitated; the solution is then cooled to -5 DEG C. or below and further alcohol is added to give a volume concentration of at least 61 per cent, when more hyaluronidase is precipitated and is separated at - 5 DEG C. or below. If desired, one or more intermediate additions of alcohol are made, e.g. to give a volume concentration of 50-55 per cent. The precipitated hyaluronidase-containing material may be separated after any or all of the additions of alcohol. It may then be dissolved in water and lyophilized to give a dry preparation substantially free from inactive proteinaceous materials. Examples are given of the process using acid sodium acetate as buffer and sodium chloride as electrolyte. Other suitable buffers and electrolytes are mentioned. Specifications 651,544 and 651,545 are referred to. |
| priorityDate | 1950-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.