http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-322465-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7b04f5b6a02051c94d6eaf190b751ed2 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07J9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07J75-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07J9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07J75-00 |
| filingDate | 1928-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1929-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-322465-A |
| titleOfInvention | Improvements in the production of valuable products from organised substances |
| abstract | 322,465. Johnson, J. Y., (I. G. Farbenindustrie Akt.-Ges.). June 2, 1928. Sterols &c.-Valuable products such as sterols and degradation products of albumens are obtained from organized substances by degrading the bulk of the cell walls by autolysis or by a protracted enzymatic treatment, into water insoluble products, separating the water soluble from the water insoluble products and treating the latter with a practically anhydrous organic solvent, preferably after a treatment with an alkaline agent. The enzymes required may be produced by adding cultures of bacteria forming enzymes capable of degrading the cell walls of the initial material, which bacteria have been previously grown on the organized substances to be acted upon. Thus, the autolysis of the cells of yeast may be performed by mixing the yeast with cultures of bacillus subtiles, bacillus mycoides or bacillus mesentericus which have been grown on autolyzed yeast. By-products such as tyrosine, or purine derivatives such as xanthine and the like, may be recovered. In examples, (1) yeast is subjected to a fargoing autolysis which may be initiated by acetic ester and extracted with an excess of alcohol to obtain the ergosterol ester which is saponified and the ergosterol ethered out. (2) Yeast is autolyzed with a cell poison such as ethyl acetate or chloroform and mixed with a culture of bacillus subtilis grown on autolyzed yeast. Part of the liquified mass is separated mechanically and the residue is worked up as before for ergosterol. (3) Yeast is liquefied by ethyl acetate until tyrosine begins to separate out, and the liquid autolysate free from tyrosine is centrifuged to separate the undissolved fraction which contains the whole of the ergosterol ester. Other valuable substances such as leucine can be recovered by the usual methods and also all the usual cleavage products of yeast. The ergosterol may be recovered from the separated crude product by esterification with acetic anhydride and saponifying. |
| priorityDate | 1928-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.