| abstract |
The enzyme kit includes a first reagent and a second reagent, where the first reagent includes pronase, and the second reagent includes papain. The single-cell suspension obtained by the method of the present disclosure has a high survival rate and a low aggregation rate, which is conducive to subsequent sorting and single-cell sequencing. The first or second reagents may also include HAG medium, made of Hibernate A, Actinomycin D, and GlutaMAX 1. The method may include dividing the tissue sample into two parts, digesting the first part with the first reagent and the second part with the second reagent, and mixing the first and second digested solution in the same HAG medium. There may be additional filtration and centrifugation steps. |