| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3e24a4521c4eb166a7962aa5e0ae4159
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e4775991c8367272640e3c9e4b6516e9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_58fa3bc54405309154ad7499b7f84c3e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bb293b5b2c4975dcdea8ca9add459223 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2560-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-912
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-7028 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5091
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6803
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57415
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57488
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B40-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6842 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-574 |
| filingDate |
2017-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f3d9fd28ea49507a053ed2905c1d63ef
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_23be581b325ff90c1084a1837132776e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0f5776068d635814e06fb3ccd37aaf81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8f1f4870e7bbe42cc8fd7771ac642552 |
| publicationDate |
2019-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
GB-2574785-A |
| titleOfInvention |
Method and system for identifying membrane proteins on extracellular vesicles |
| abstract |
Disclosed is method of isolating extracellular vesicles and identifying membrane proteins therefrom. The method includes providing human plasma and/or serum; separating lipoproteins and extracellular vesicles from the human plasma and/or serum by a density gradient preparation, collecting the extracellular vesicles from the separated lipoproteins and extracellular vesicles; isolating and purifying the collected extracellular vesicles by using size exclusion chromatography; treating the isolated and purified extracellular vesicles with an aqueous solution to obtain membranes of the extracellular vesicles, wherein the aqueous solution has a pH in a range of 9 to 14; adding salt in a concentration range between 0.5 – 2.0 M to the aqueous solution; isolating the membranes from the treated extracellular vesicles and identifying proteins on the isolated membranes by employing mass spectrometer. Also disclosed are associated methods of identifying breast cancer specific membrane proteins, systems for identifying biomarkers for breast cancer using healthy plasma and carcinogenic breast tissues, and kits for capturing extracellular vesicles and detecting breast cancer associated markers, wherein the kits comprise epitope specific binding agents and detection agents. |
| priorityDate |
2017-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |