Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_872dd7cd609eb2a937dcfd303d521798 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-15051 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-15043 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1017 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-0091 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-86 |
filingDate |
2017-08-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d320c134b345b1847053372f5424decb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e71adbbfbc5f176156f36a92a8040b55 |
publicationDate |
2018-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
GB-2558698-A |
titleOfInvention |
Method for purifying viral vectors |
abstract |
A method of purifying viral vectors from cell culture comprising filtering the cell culture using diatomaceous earth (DE). The method may comprise adding DE at a concentration of 2-4 g DE/L/%, to a HEK293 cell culture expressing viral vectors, such as lentiviruses or adeno-associated viruses (AVV), followed by filtration steps comprising a 0.45 µm filter. Preferably the amount of DE is 3 g/L/% per wet cell weight. Also claimed is a method of determining the optimal amount of DE to use comprising determining the percentage of wet cell weight in the cell culture and calculating the amount of DE to use when purifying the viral vectors from the cell culture so that the amount of DE is 2-4 g DE/L/%. |
priorityDate |
2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |