| abstract |
The invention relates to retroviral producer cell comprising nucleic acid sequences encoding: gag and pol proteins; envelope protein or a functional substitute thereof; and the RNA genome of the retroviral vector particle, wherein said nucleic acid sequences are all located at a single locus within the retroviral producer cell genome. Said cells may further comprise the auxiliary gen rev or analogue thereof. The retrovirus may be selected from lentivirus, alpha-retrovirus, gamma-retrovirus or foamy-retrovirus, including HIV, SIV, FIV, EIAV or VISNA. The producer cell is typically a mammalian cell. |