| abstract |
A compound of Formula (III): Wherein Z1 is either O or S; and R3 is select from hydrogen, bromine, a substituted or unsubstituted aryl, arylalkyl, arylalkenyl, arylalkynyl; or a alkyl, alkenyl or alkynyl optionally substituted with an alicyclic group; an alicyclic group; or a heterocyclic group. A fluorescent protein comprising a compound of Formula (III), an apoprotein of a calcium-binding photoprotein and a calcium ion, or a divalent or trivalent ion replaceable for the calcium ion is also provided. A method of forming the fluorescent protein comprises contacting a compound of Formula (III) with an apoprotein of a calcium-binding photoprotein, optionally in the presence of a chelating agent and/or a reducing agent. A method for analyzing a physiological function or enzyme activity comprises performing bioluminescence resonance energy transfer using the produced calcium-binding photoprotein. |