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titleOfInvention Infrared imaging of biological material
abstract A method of characterising a tissue sample; used for example in breast cancer pathology imaging. Infrared absorption data is obtained from the sample at selected wavelengths, and a measure of energy or power absorbed by an amide and a phosphate moiety is determined 30. Each measure may be an area under an absorption peak. A ratio of the measures is used to establish a histology index. Each measure may correspond to a pixel in a two dimensional array, used to map the histological index 38. A pixel replacement procedure 33-36, using a histogram of measured absorption values, may be used to substitute outlier pixels with an average value of adjacent pixels. Each pixel may be classified as indicating benign or cancerous tissue, with a grading.
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