| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8a80b9bef8381cbd855114f4801ba7df |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-8831
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-02 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N24-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-7266
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-8679
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-7233 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N24-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C02F1-42
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate |
2013-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d9714652681751182cbc4f03efeff39c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f3580c5d9da69fa69983d44ec78cc4f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_edfeab1fdb3cc64038f7ea5887c9f6d1 |
| publicationDate |
2014-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
GB-2513805-A |
| titleOfInvention |
Methods for quantifying polypeptides using mass spectrometry |
| abstract |
A method for identifying a polypeptide a specimen can include (i) treating a specimen suspected of comprising an insulin with a base; (ii) extracting a first fraction of the treated specimen by solid phase extraction using a mixed mode or polymeric reversed-phase media and a first solvent comprising an acid; (iii) separating a component of the first fraction by liquid chromatography using a chromatographic surface including a hydrophobic surface group and one or more ionizable modifiers, and a second solvent comprising an acid; and (iv) analyzing the component of the first fraction by mass spectroscopy, thereby identifying the polypeptide, if present, using a signal corresponding to a sequence fragment ion from the polypeptide. The signal can correspond to an intact multiply charged precursor fragment selected in a first quadrupole and its corresponding sequence fragment ion selected in a final quadrupole. |
| priorityDate |
2012-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |