http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2485275-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3a6d4c820ab26e0395f6e57dd3ac7a80 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1252 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 |
| filingDate | 2011-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d3b5605420e773fa94dddf7a6b63fb4 |
| publicationDate | 2012-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-2485275-A |
| titleOfInvention | Strand displacement activity of modified polymerases |
| abstract | A method for detecting or amplifying a target nucleic acid in a reaction involving primer extension and strand displacement comprises the use of a modified DNA polymerase derived from thermophilic bacteria of the Deinococcus-Thermus group, wherein the modified polymerase exhibits strand displacement activity but does not possess 5รข nuclease activity, Preferably the polymerase is a modified Taq DNA polymerase. The method preferably comprises the use of 2 forward primers and one reverse primer, and may be a PCR or PCDR (polymerase chain displacement reaction) amplification. Preferably the method is asymmetric, such that the reverse primer is in excess, and the reaction product may be detected by probes carrying a fluorescent reporter and a quencher. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3778891-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3450558-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016033315-A3 |
| priorityDate | 2010-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 134.