http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2466177-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b27db37f347109bc2490eed3a4189427 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2795-00051 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2795-00032 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-76 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-76 |
| filingDate | 2008-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06e79fb0df0ada77f66836b0be1ca4b8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c5ff86e2435aec53b7b8ae04478cb69d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_29dbdc50add31d58787b4faf72ef14af |
| publicationDate | 2010-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-2466177-A |
| titleOfInvention | Bacteriophage selection and breeding |
| abstract | A method of selectively breeding bacteriophages comprises incubating phage obtained from natural sources with bacterial host, removing the host cells and plating out the phages on a bacterial lawn, assessing the plaques and isolating phage from the plaques showing the greatest activity, culturing the isolated phage with host bacteria and adding a viricidal medium to remove free phage, plating the viricidally-treated culture onto a host bacterial lawn, and identifying and selecting plaques showing the most virulent phage activity. The methods described do not use genetic manipulation techniques. The viricidal medium preferably comprises pomegranate rind extract, an iron salt (preferably ferrous sulphate), and a polysorbate surfactant (preferably Tween (RTM) 20). A method of modifying phage host sensitivity is also disclosed, in which phages are incubated with one or more of a chelating agent (such as EDTA), a detergent (preferably Tween (RTM) 20), a lantibiotic such as Nisin A, an enzyme such as lysozyme and/or a cell wall-destroying agent. |
| priorityDate | 2008-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 120.