| abstract |
Short hairpin RNA (shRNA) polynucleotides comprising an RNA sequence comprising a first stretch of 19 consecutive nucleotides and a second stretch of 19 consecutive nucleotides, which is the reverse-complement of the first stretch, wherein in said second stretch: a) in the 5'-3' direction, the last nucleotide is a C nucleotide, b) no stretches of four or more consecutive identical nucleotides are present, c) G+C is 30-70% and d) in the 5'-3' direction, consecutive nucleotides 1-19 are homologous to an RNA molecule. Also claimed are vectors comprising such molecules, and methods of preparing such vectors. |