| abstract |
The present invention discloses a method of making a three-dimensional porous growth surface hydrogel comprising apply to a surface an anionic polysaccharide material, preferably alginic acid or derivatives. Divalent cations, preferably Ca2<+>, at a concentration of at least 2.3mM may be incorporated in the hydrogel, applied to the surface or supplemented in the culture or any combinations thereof. The growth media may be a polypropylene or nylon mesh and shaped. The hydrogel surface may also be coated with a polycation such as poly-l-lysine, polyarginine, polyethyleneimine, poly-D-ornithine, poly-l-ornithine. |