| abstract |
Agarose is used to provide a coating having a cytophobic effect on a support used for screening an array of polymer samples for cell-binding properties. Polymers for cell-binding properties are screened by forming a polymer library, such as a library of polyurethanes, by parallel synthesis, at least partially characterising the library members, forming an array of the library members on a support such as a glass slide, incubating the microarray with a cell suspension and observing the microarray to determine the binding activity of library members with specific cells. The method is used to identify polymers that bind to ostoprogenitor cells, so that the polymers can be used as a substrate for contact with unselected human bone marrow mononuclear cell preparations for selective immobilisation of ostoprogenitor cells. Suitable polyurethanes are made from polyols such as poly(propylene glycol), poly(tetramethylene glycol, poly [1, 6- hexanediol/neopentyl glycol/diethylene glycol-alt-(adipic acid)] diol, poly[1, 6- hexanediol/neopentyl glycol-alt- (adipic acid)] diol, isocyanate such as 1 ,6-diisocyanohexane, 4, 4' methylenebis(phenylisocyanate), 4-methyl- 1,3, phenylene diisocyanate, 1, 4 diisocyanobenzene, 4, 4' methylenebis(cyclohexylisocyanate) and 1, 3 bis(isocyanatomethyl)cyclohexane and chain extenders such as 1, 4 butanediol, ethylene glycol, propylene glycol, ethylene diamine, 3-dimethylamino-1,2-propanediol, 2-nitro-2- methyl-1, 3 propanediol, 2,2,3, 3, 4, 4, 5, 5 -octofluoro-1,6, hexanediol, and diethyl bis(hydroxymethyl)malonate. |