http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2421243-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_37b416a8c0d2b0f1c3d6026d932027af |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6809 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6809 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2003-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a6f827d58e9c0db49f2be0c771b84f3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a67e933eac587b4435999e4a2a360e5a |
publicationDate | 2006-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | GB-2421243-A |
titleOfInvention | Database generation |
abstract | A method of generating a database comprising a plurality of records, each record comprising an indication whether a gene is expressed by a particular cell, the method comprising steps of: (a) providing a first nucleic acid sequence; (b) linking the first nucleic acid sequence to an linker sequence to form a linked nucleic acid, in which the linker sequence comprises: (i) a first recognition site for a first nucleic acid cleavage enzyme that allows cleavage of the first nucleic acid sequence at a site distant from the first recognition site, and (ii) a second recognition site for a second nucleic acid cleavage enzyme that allows nucleic acid cleavage at a site distant from the second recognition site, said cleavage site located at a position within or about the first recognition site; in which the linked nucleic acid has the structure: 5' - second recognition site - first recognition site - first nucleic acid - 3' (c) cleaving the linked nucleic acid with the first nucleic acid cleavage enzyme to provide: (i) a linked tag comprising the linker sequence linked to a nucleotide sequence tag representative of the first nucleic acid sequence and comprising a terminal portion thereof; and (ii) a second nucleic acid sequence comprising a remainder portion of the first nucleic acid; (d) detecting the presence, sequence or identity of the linked tag or the nucleotide sequence tag to provide an indication of an instance of gene expression; and storing an indication of expression of the gene as a record in the database. |
priorityDate | 2002-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 132.